Developing clinically relevant biomarkers in inflammatory arthritis: A multiplatform approach for serum candidate protein discovery
Identifieur interne : 000E20 ( Main/Exploration ); précédent : 000E19; suivant : 000E21Developing clinically relevant biomarkers in inflammatory arthritis: A multiplatform approach for serum candidate protein discovery
Auteurs : Angela Mcardle [Irlande (pays)] ; Aisha Qasim Butt [Irlande (pays)] ; Agnes Szentpetery [Irlande (pays)] ; Wilco De Jager [Pays-Bas] ; Sytze De Roock [Pays-Bas] ; Oliver Fitzgerald [Irlande (pays)] ; Stephen R. Pennington [Irlande (pays)]Source :
- PROTEOMICS – Clinical Applications [ 1862-8346 ] ; 2016-06.
Abstract
Purpose: To identify candidate biomarkers that have the potential to distinguish between patients with psoriatic arthritis (PsA) or rheumatoid arthritis (RA) and explore the value of combining different protein discovery platforms for the development of a multiplexed protein biomarker panel. Experimental design: Serum samples from 32 patients (PsA; n = 16 and RA; n = 16) defined as active, early onset, and treatment naïve were analyzed using unbiased label‐free LC‐MS/MS, a microsphere bead‐based immunoassay (Luminex xMAP) and an aptamer‐based assay (SOMAscan). Results: LC‐MS/MS was used to quantify 324 proteins, while the Luminex xMAP targeted 48 proteins and SOMAscan supported the measurement of 1129 proteins. The combined data from these techniques gave reproducible quantification of 1501 proteins in total. Of these, 42 (LC‐MS/MS), 3 (Luminex xMAP), and 127 (SOMAscan) proteins were found to be differentially expressed between PsA and RA (p < 0.05). Conclusion and clinical relevance: Using three different and potentially complementary proteomic platforms we identified a total of 172 proteins that are differentially expressed in patients with PsA compared to RA. These proteins collectively represent candidates for inclusion in a protein signature that could be developed as a diagnostic test to discriminate patients with PsA from RA and therefore be of clinical utility.
Url:
DOI: 10.1002/prca.201500046
Affiliations:
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Experimental design: Serum samples from 32 patients (PsA; n = 16 and RA; n = 16) defined as active, early onset, and treatment naïve were analyzed using unbiased label‐free LC‐MS/MS, a microsphere bead‐based immunoassay (Luminex xMAP) and an aptamer‐based assay (SOMAscan). Results: LC‐MS/MS was used to quantify 324 proteins, while the Luminex xMAP targeted 48 proteins and SOMAscan supported the measurement of 1129 proteins. The combined data from these techniques gave reproducible quantification of 1501 proteins in total. Of these, 42 (LC‐MS/MS), 3 (Luminex xMAP), and 127 (SOMAscan) proteins were found to be differentially expressed between PsA and RA (p < 0.05). Conclusion and clinical relevance: Using three different and potentially complementary proteomic platforms we identified a total of 172 proteins that are differentially expressed in patients with PsA compared to RA. These proteins collectively represent candidates for inclusion in a protein signature that could be developed as a diagnostic test to discriminate patients with PsA from RA and therefore be of clinical utility.</div></front></TEI><affiliations><list><country><li>Irlande (pays)</li><li>Pays-Bas</li></country><region><li>Leinster</li><li>Utrecht (province)</li></region><settlement><li>Dublin</li><li>Utrecht</li></settlement><orgName><li>University College Dublin</li></orgName></list><tree><country name="Irlande (pays)"><region name="Leinster"><name sortKey="Mcardle, Angela" sort="Mcardle, Angela" uniqKey="Mcardle A" first="Angela" last="Mcardle">Angela Mcardle</name></region><name sortKey="Fitzgerald, Oliver" sort="Fitzgerald, Oliver" uniqKey="Fitzgerald O" first="Oliver" last="Fitzgerald">Oliver Fitzgerald</name><name sortKey="Fitzgerald, Oliver" sort="Fitzgerald, Oliver" uniqKey="Fitzgerald O" first="Oliver" last="Fitzgerald">Oliver Fitzgerald</name><name sortKey="Pennington, Stephen R" sort="Pennington, Stephen R" uniqKey="Pennington S" first="Stephen R." last="Pennington">Stephen R. Pennington</name><name sortKey="Pennington, Stephen R" sort="Pennington, Stephen R" uniqKey="Pennington S" first="Stephen R." last="Pennington">Stephen R. Pennington</name><name sortKey="Pennington, Stephen R" sort="Pennington, Stephen R" uniqKey="Pennington S" first="Stephen R." last="Pennington">Stephen R. Pennington</name><name sortKey="Qasim Butt, Aisha" sort="Qasim Butt, Aisha" uniqKey="Qasim Butt A" first="Aisha" last="Qasim Butt">Aisha Qasim Butt</name><name sortKey="Szentpetery, Agnes" sort="Szentpetery, Agnes" uniqKey="Szentpetery A" first="Agnes" last="Szentpetery">Agnes Szentpetery</name></country><country name="Pays-Bas"><region name="Utrecht (province)"><name sortKey="De Jager, Wilco" sort="De Jager, Wilco" uniqKey="De Jager W" first="Wilco" last="De Jager">Wilco De Jager</name></region><name sortKey="De Jager, Wilco" sort="De Jager, Wilco" uniqKey="De Jager W" first="Wilco" last="De Jager">Wilco De Jager</name><name sortKey="De Roock, Sytze" sort="De Roock, Sytze" uniqKey="De Roock S" first="Sytze" last="De Roock">Sytze De Roock</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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